Directly lighting up RNA G-quadruplexes from test tubes to living human cells

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	Directly lighting up RNA G-quadruplexes from test tubes to living human cells
	Xu, Shujuan 1,2; Li, Qian 1; Xiang, Junfeng 1; Yang, Qianfan 1; Sun, Hongxia 1; Guan, Aijiao 1; Wang, Lixia 1; Liu, Yan 1; Yu, Lijia 1,2; Shi, Yunhua 1,2
刊名	NUCLEIC ACIDS RESEARCH
	2015-11-16
卷号	43 期号:20 页码:9575-9586
英文摘要	RNA G-quadruplexes (G4s) are one of the key components of the transcriptome that act as efficient post-transcriptional regulatory elements in living cells. To conduct further studies of the unique biological functions of RNA G4s, techniques need to be developed that can efficiently recognize RNA G4 structures under various conditions, in fixed cells and living cells, as well as in vitro. This paper presents the development of such a method, a new technique using a cyanine dye called CyT, which can detect both canonical and non-canonical RNA G4 structures from test tubes to living human cells. The ability of CyT to distinguish between G4 and nonG4 RNA offers a promising tool for future RNA G4-based biomarker discovery and potential diagnostic applications.
收录类别	SCI
语种	英语
公开日期	2016-02-25
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/29514]
专题	化学研究所_分子动态与稳态结构实验室
作者单位	1.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Nat Lab Mol Sci,Ctr Mol Sci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Shujuan,Li, Qian,Xiang, Junfeng,et al. Directly lighting up RNA G-quadruplexes from test tubes to living human cells[J]. NUCLEIC ACIDS RESEARCH,2015,43(20):9575-9586.
APA	Xu, Shujuan.,Li, Qian.,Xiang, Junfeng.,Yang, Qianfan.,Sun, Hongxia.,...&Tang, Yalin.(2015).Directly lighting up RNA G-quadruplexes from test tubes to living human cells.NUCLEIC ACIDS RESEARCH,43(20),9575-9586.
MLA	Xu, Shujuan,et al."Directly lighting up RNA G-quadruplexes from test tubes to living human cells".NUCLEIC ACIDS RESEARCH 43.20(2015):9575-9586.