A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E

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	A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E
	Li AL; Li HW; Jin G(靳刚); Xiu RJ
刊名	CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
	2003
通讯作者邮箱	xiurj@yahoo.com.cn
卷号	29 期号:3-4 页码:383-390
关键词	tumor conditioned medium endothelium cell cycle 2-DE Western blot
ISSN号	1386-0291
通讯作者	Xiu, RJ (reprint author), Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China.
产权排序	Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China; Peking Union Med Coll, Beijing 100005, Peoples R China; Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China
中文摘要	This study was designed to comprehensively analyze the differential expression of proteins from human umbilical vein endothelial cells (HUVECs) exposed to tumor conditioned medium (TCM) and to identify the key regulator in the cell cycle progression. The HUVECs were exposed to TCM from breast carcinoma cell line MDA-MB-231, then their cell cycle distribution was measured by flow cytometer (FCM). The role of protein in cell cycle progression was detected via two-dimensional polyacrylamide gel electrophoresis (2-DE) and western blotting. Following the stimulation of TCM, HUVECs showed a more cells in the S phase than did the negative control group (ECGF-free medium with 20% FBS), but the HUVECs' level was similar to the positive control group (medium with 25 mug/ml ECGF and 20% FBS). Increased expression of cyclin D-1/E and some changes in other related proteins occurred after incubation with TCM. From our results, we can conclude that breast carcinoma cell line MDA-MB-231 may secrete soluble pro-angiogenic factors that induce the HUVEC angiogenic switch, during which the expression of cell cycle regulator cyclin D-1/E increases and related proteins play an important role in this process.
类目[WOS]	Hematology ; Peripheral Vascular Disease
研究领域[WOS]	Hematology ; Cardiovascular System & Cardiology
关键词[WOS]	GROWTH-FACTOR ; CANCER-CELLS ; AGENT TNP-470 ; IN-VITRO ; ANGIOGENESIS ; ANTISENSE ; D1 ; INHIBITION ; P21(WAF1/CIP1) ; TUMORIGENICITY
收录类别	SCI
语种	英语
WOS记录号	WOS:000188240800032
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/58477]
专题	力学研究所_力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Li AL,Li HW,Jin G,et al. A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E[J]. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2003,29(3-4):383-390.
APA	Li AL,Li HW,靳刚,&Xiu RJ.(2003).A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E.CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,29(3-4),383-390.
MLA	Li AL,et al."A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E".CLINICAL HEMORHEOLOGY AND MICROCIRCULATION 29.3-4(2003):383-390.