The Mitogenic and Anti-Apoptotic Activity of Tumor Conditioned Medium on Endothelium

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	The Mitogenic and Anti-Apoptotic Activity of Tumor Conditioned Medium on Endothelium
	Li AL; Li HW; Zhang J; Jin G(靳刚); Xiu RJ
刊名	CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
	2003
通讯作者邮箱	xiurj@yahoo.com.cn
卷号	29 期号:3-4 页码:375-382
关键词	tumor conditioned medium endothelium proliferation apoptosis cell cycle
ISSN号	1386-0291
通讯作者	Xiu, RJ (reprint author), Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China.
产权排序	Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China; Peking Union Med Coll, Beijing 100005, Peoples R China; Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China
中文摘要	This study was designed to observe the effect of tumor conditioned medium (TCM) on the proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs). HUVECs were exposed to TCM from breast carcinoma cell line MDA-MB-231, then we measured their proliferation, apoptosis and cell cycle distribution by MTT and flow cytometery (FCM). Following the stimulation of TCM, HUVECs showed higher pro-mitogenic and anti-apoptotic ability than did the negative control group (ECGF-free medium with 20% FBS), but a similar ability to the positive control group (medium with ECGF and 20% FBS). From these results, we can conclude that breast carcinoma cell line MDA-MB-231 could secret soluble pro-angiogenic factors that induce HUVEC angiogenic switching, including cell cycle progression, proliferation and growth. The role and character of these factors remain to be further studied.
类目[WOS]	Hematology ; Peripheral Vascular Disease
研究领域[WOS]	Hematology ; Cardiovascular System & Cardiology
关键词[WOS]	GROWTH-FACTOR ; IN-VITRO ; ANGIOGENESIS ; CELLS ; IDENTIFICATION ; MODULATION ; EXPRESSION ; CULTURE
收录类别	SCI
语种	英语
WOS记录号	WOS:000188240800031
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/58476]
专题	力学研究所_力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Li AL,Li HW,Zhang J,et al. The Mitogenic and Anti-Apoptotic Activity of Tumor Conditioned Medium on Endothelium[J]. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2003,29(3-4):375-382.
APA	Li AL,Li HW,Zhang J,靳刚,&Xiu RJ.(2003).The Mitogenic and Anti-Apoptotic Activity of Tumor Conditioned Medium on Endothelium.CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,29(3-4),375-382.
MLA	Li AL,et al."The Mitogenic and Anti-Apoptotic Activity of Tumor Conditioned Medium on Endothelium".CLINICAL HEMORHEOLOGY AND MICROCIRCULATION 29.3-4(2003):375-382.