Dynamical and Microrheological Analysis of Amyloplasts in the Plant Root Gravity-Sensing Cells

	Dynamical and Microrheological Analysis of Amyloplasts in the Plant Root Gravity-Sensing Cells
	Zheng ZY(郑中玉); Zou JJ; Li HH; Xue S; Le J; Wang YR(王育人)
刊名	MICROGRAVITY SCIENCE AND TECHNOLOGY
	2015-09
通讯作者邮箱	zzy@imech.ac.cn ; zoujunjie@ibcas.ac.cn ; lihanhai@imech.ac.cn ; xueshan1984@yeah.net ; lejie@ibcas.ac.cn ; yurenwang@imech.ac.cn
卷号	27 期号:6 页码:485-493
关键词	Plant gravitropism Gravity sensing Amyloplasts Columella cells Microrheology Diffusion Cage-confinement
ISSN号	0938-0108
通讯作者	Zheng, ZY (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China.
产权排序	[Zou, Junjie; Xue, Shan; Le, Jie] Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China; [Zheng, Zhongyu; Li, Hanhai; Wang, Yuren] Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China
中文摘要	Gravitropism in plants is one of the most controversial issues. In the most wildly accepted starch-statolith hypothesis the sedimentation movement of amyloplasts in the gravisensing columella cells primarily triggers the asymmetric distribution of auxin which leads to the differential growth of the plant root. It has been gradually recognized that the inhomogeneous structures in statocytes arising from intracellular components such as cytoskeletons significantly affect the complex movements of amyloplasts and the final gravimorphogenesis. In this letter, we implement a diffusive dynamics measurement and inplanta microrheological analysis of amyloplasts in the wild-type plants and actin cytoskeleton mutants for the first time. We found that the intracellular environment of columella cells exhibits the spatial heterogeneity and the cage-confinement on amyloplasts which is the typically characteristics in colloidal suspensions. By comparing the distinct diffusive dynamics of amyloplasts in different types of plants with the behaviors of colloidal systems in different states, we quantitatively characterized the influence of the actin organization dominated intracellular envoronments on the amyloplast movements. Furthermore, the cage-confinement strength was measured by calculating the spatial fluctuation of local apparent viscosity within the columella cells. Finally, a linear association between the initial mechanical stimulation in the columella cells the subsequent intercellular signal transduction and the final gravity response was observed and a possible gravity sensing mechanism was suggested. It suggests the existence of a potential gravity-sensing mechanism that dictates a linear frustration effect of the actin cytoskeleton on the conversion of the mechanical stimulation of amyloplasts into gravitropic signals.
分类号	二类/Q2
类目[WOS]	Engineering, Aerospace ; Thermodynamics ; Mechanics
研究领域[WOS]	Engineering ; Thermodynamics ; Mechanics
关键词[WOS]	ACTIN CYTOSKELETON ; AUXIN ; GRAVITROPISM ; DISRUPTION ; TRANSPORT ; NETWORKS ; COMPLEX
收录类别	SCI ; EI
原文出处	http://dx.doi.org/10.1007/s12217-015-9445-x
语种	英语
WOS记录号	WOS:000366103800010
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/58367]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Zheng ZY,Zou JJ,Li HH,et al. Dynamical and Microrheological Analysis of Amyloplasts in the Plant Root Gravity-Sensing Cells[J]. MICROGRAVITY SCIENCE AND TECHNOLOGY,2015,27(6):485-493.
APA	郑中玉,Zou JJ,Li HH,Xue S,Le J,&王育人.(2015).Dynamical and Microrheological Analysis of Amyloplasts in the Plant Root Gravity-Sensing Cells.MICROGRAVITY SCIENCE AND TECHNOLOGY,27(6),485-493.
MLA	郑中玉,et al."Dynamical and Microrheological Analysis of Amyloplasts in the Plant Root Gravity-Sensing Cells".MICROGRAVITY SCIENCE AND TECHNOLOGY 27.6(2015):485-493.