One-pot one-cluster synthesis of fluorescent and bio-compatible Ag-14 nanoclusters for cancer cell imaging

CORC > 化学研究所 > 中国科学院化学研究所 > 分子动态与稳态结构实验室

	One-pot one-cluster synthesis of fluorescent and bio-compatible Ag-14 nanoclusters for cancer cell imaging
	Yang, Jie 1,2; Xia, Nan 1; Wang, Xinan 3; Liu, Xianhu 4; Xu, An 3; Wu, Zhikun 1; Luo, Zhixun 4
刊名	NANOSCALE
	2015
卷号	7 期号:44 页码:18464-18470
英文摘要	Small-molecule-protected silver nanoclusters have smaller hydrodynamic diameter, and thus may hold greater potential in biomedicine application compared with the same core-sized, macromolecule (i.e. DNA)-protected silver nanoclusters. However, the live cell imaging labeled by small-molecule-protected silver nanoclusters has not been reported until now, and the synthesis and atom-precise characterization of silver nanoclusters have been challenging for a long time. We develop a one-pot one-cluster synthesis method to prepare silver nanoclusters capped with GSH which is bio-compatible. The as-prepared silver nanoclusters are identified to be Ag-14(SG)(11) (abbreviated as Ag-14, SG: glutathione) by isotope-resolvable ESI-MS. The structure is probed by 1D NMR spectroscopy together with 2D COSY and HSQC. This cluster species is fluorescent and the fluorescence quantum yield is solvent-dependent. Very importantly, Ag-14 was successfully applied to label lung cancer cells (A549) for imaging, and this work represents the first attempt to image live cells with small-molecule-protected silver nanoclusters. Furthermore, it is revealed that the Ag-14 nanoclusters exhibit lower cytotoxicity compared with some other silver species (including silver salt, silver complex and large silver nanoparticles), and the explanation is also provided. The comparison of silver nanoclusters to state-of-the-art labeling materials in terms of cytotoxicity and photobleaching lifetime is also conducted.
收录类别	SCI
语种	英语
公开日期	2015-12-29
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/29295]
专题	化学研究所_分子动态与稳态结构实验室
作者单位	1.Chinese Acad Sci, Inst Solid State Phys, Anhui Key Lab Nanomat & Nanotechnol, Key Lab Mat Phys, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China 4.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Yang, Jie,Xia, Nan,Wang, Xinan,et al. One-pot one-cluster synthesis of fluorescent and bio-compatible Ag-14 nanoclusters for cancer cell imaging[J]. NANOSCALE,2015,7(44):18464-18470.
APA	Yang, Jie.,Xia, Nan.,Wang, Xinan.,Liu, Xianhu.,Xu, An.,...&Luo, Zhixun.(2015).One-pot one-cluster synthesis of fluorescent and bio-compatible Ag-14 nanoclusters for cancer cell imaging.NANOSCALE,7(44),18464-18470.
MLA	Yang, Jie,et al."One-pot one-cluster synthesis of fluorescent and bio-compatible Ag-14 nanoclusters for cancer cell imaging".NANOSCALE 7.44(2015):18464-18470.