Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases

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	Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases
	Zhang, Chenxu 1,2; Zhang, Rongyue 3; Li, Qiang 1; Huang, Yongdong 1; Zhao, Lan 1; Su, Zhiguo 1; Gong, Fangling 1; Lv, Zheng 4; Song, Hongyan 4; Li, Wei 4
刊名	SEPARATION AND PURIFICATION TECHNOLOGY
	2015-11-05
卷号	154 期号:NOV 页码:351-358
关键词	Octreotide Reversed-phase Separation Poly(styrene-co-divinylbenzene)
ISSN号	1383-5866
英文摘要	Octreotide, a disulfide-cyclized eight amino acids long analogue of somatostatin, has been used to treat the symptoms associated with metastatic carcinoid tumors (flushing and diarrhea), and vasoactive intestinal peptide secreting adenomas (watery diarrhea). As composed by D-amino acids, C-terminal alcohol, and disulfide bond, octreotide can be only prepared using chemistry synthesis method, which increases difficulty of the traditional reversed-phase separation. In this paper, we report a very fast separation of octreotide from synthetic peptide crude mixtures on a poly(styrene co-divinylbenzene) (PSt)-based reversed phases prepared by a quick membrane emulsification process. In general, fourier-transformed infrared resonance (FT-IR) spectroscopy, reversed-phase high performance liquid chromatography (HPLC) combined with electrospray ionisation mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR) spectroscopy are routinely used for analyses of resultant peptides. Under the optimized conditions, the octreotide is purified from 42.89% to 99.99%. Consequently, the poly(styrene co-divinylbenzene)-based phase can provide more flexibility to improve separation efficiency for the octreotide from the synthetic peptide crude mixtures. (C) 2015 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Chemical
研究领域[WOS]	Engineering
关键词[WOS]	AMINO-ACID ; PURIFICATION ; POLYSTYRENE ; PROTEINS ; HPLC ; SILICA ; COLUMNS ; RESINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000364245500043
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/19808]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China 3.Beijing Inst Petrochem Technol, Dept Chem Engn, Beijing 102617, Peoples R China 4.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Chenxu,Zhang, Rongyue,Li, Qiang,et al. Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2015,154(NOV):351-358.
APA	Zhang, Chenxu.,Zhang, Rongyue.,Li, Qiang.,Huang, Yongdong.,Zhao, Lan.,...&Ma, Guanghui.(2015).Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases.SEPARATION AND PURIFICATION TECHNOLOGY,154(NOV),351-358.
MLA	Zhang, Chenxu,et al."Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases".SEPARATION AND PURIFICATION TECHNOLOGY 154.NOV(2015):351-358.