Comparison of covalent and physical immobilization of lipase in gigaporous polymeric microspheres

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 生化工程国家重点实验室

	Comparison of covalent and physical immobilization of lipase in gigaporous polymeric microspheres
	Wang, Weichen 1,2; Zhou, Weiqing 1; Li, Juan 1; Hao, Dongxia 1; Su, Zhiguo 1,3; Ma, Guanghui 1,3
刊名	BIOPROCESS AND BIOSYSTEMS ENGINEERING
	2015-11-01
卷号	38 期号:11 页码:2107-2115
关键词	Gigaporous microspheres Lipase immobilization Physical adsorption Covalent binding Hydrophobicity
ISSN号	1615-7591
英文摘要	Lipase (EC 3.1.1.3) is a versatile enzyme which has been widely used in ester-reaction industries. We have previously discovered that gigaporous polystyrene (PST) microspheres can be used as a novel immobilization carrier for lipase. In this work, a series of gigaporous microspheres with different densities of epoxy group including poly(glycidyl methacrylate) (PGMA) and poly(styrene-co-glycidyl methacrylate) [P(ST-GMA)] were evaluated as lipase immobilization carriers, which were also compared with gigaporous PST microspheres and the commercial immobilized lipase Novozym 435. Lipase immobilized in gigaporous PGMA microspheres showed the highest activity yield, reusability, and stability as well as the best affinity for the substrate. The characterizations of adsorption curves, the change of epoxy group amounts, and hydrophobic-hydrophilic properties of the microspheres were carried out to investigate the interaction between lipase molecules and carriers. It was found that covalent binding played a key role in improving the properties of lipase immobilized in gigaporous PGMA microspheres.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	MICELLES SWELLING METHOD ; CANDIDA-RUGOSA LIPASE ; ENZYME IMMOBILIZATION ; POLYESTER SYNTHESIS ; ANTARCTICA ; ADSORPTION ; STABILITY ; SUPPORT ; SILICA ; HYDROLYSIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000363022500006
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/19740]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
推荐引用方式 GB/T 7714	Wang, Weichen,Zhou, Weiqing,Li, Juan,et al. Comparison of covalent and physical immobilization of lipase in gigaporous polymeric microspheres[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2015,38(11):2107-2115.
APA	Wang, Weichen,Zhou, Weiqing,Li, Juan,Hao, Dongxia,Su, Zhiguo,&Ma, Guanghui.(2015).Comparison of covalent and physical immobilization of lipase in gigaporous polymeric microspheres.BIOPROCESS AND BIOSYSTEMS ENGINEERING,38(11),2107-2115.
MLA	Wang, Weichen,et al."Comparison of covalent and physical immobilization of lipase in gigaporous polymeric microspheres".BIOPROCESS AND BIOSYSTEMS ENGINEERING 38.11(2015):2107-2115.