Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus (SCI)

CORC > 寒区旱区环境与工程研究所 > 中科院寒区旱区环境与工程研究所（未分类） > 期刊论文

	Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus (SCI)
	Zhao Z. X. ; Fan B. ; Wu G. H. ; Yan X. M. ; Li Y. G. ; Zhou X. L. ; Yue H. ; Dai X. L. ; Zhu H. X. ; Tian B. ; Li J. ; Zhang Q.
刊名	Bmc Microbiology
	2014
卷号	14 期号:10
英文摘要	Background: Capripox viruses are economically important pathogens in goat and sheep producing areas of the world, with specific focus on goat pox virus (GTPV), sheep pox virus (SPPV) and the Lumpy Skin Disease virus (LSDV). Clinically, sheep pox and goat pox have the same symptoms and cannot be distinguished serologically. This presents a real need for a rapid, inexpensive, and easy to operate and maintain genotyping tool to facilitate accurate disease diagnosis and surveillance for better management of Capripox outbreaks. Results: A LAMP method was developed for the specific differential detection of GTPV and SPPV using three sets of LAMP primers designed on the basis of ITR sequences. Reactions were performed at 62 degrees C for either 45 or 60 min, and specificity confirmed by successful differential detection of several GTPV and SPPV isolates. No cross reactivity with Orf virus, foot- and-mouth disease virus (FMDV), A. marginale Lushi isolate, Mycoplasma mycoides subsp. capri, Chlamydophila psittaci, Theileria ovis, T. luwenshuni, T. uilenbergi or Babesia sp was noted. RFLP-PCR analysis of 135 preserved epidemic materials revealed 48 samples infected with goat pox and 87 infected with sheep pox, with LAMP test results showing a positive detection for all samples. When utilizing GTPV and SPPV genomic DNA, the universal LAMP primers (GSPV) and GTPV LAMP primers displayed a 100% detection rate; while the SPPV LAMP detection rate was 98.8%, consistent with the laboratory tested results. Conclusions: In summary, the three sets of LAMP primers when combined provide an analytically robust method able to fully distinguish between GTPV and SPPV. The presented LAMP method provides a specific, sensitive and rapid diagnostic tool for the distinction of GTPV and SPPV infections, with the potential to be standardized as a detection method for Capripox viruses in endemic areas.
收录类别	SCI
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.casnw.net/handle/362004/24816]
专题	寒区旱区环境与工程研究所_中科院寒区旱区环境与工程研究所（未分类）_期刊论文
推荐引用方式 GB/T 7714	Zhao Z. X.,Fan B.,Wu G. H.,et al. Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus (SCI)[J]. Bmc Microbiology,2014,14(10).
APA	Zhao Z. X..,Fan B..,Wu G. H..,Yan X. M..,Li Y. G..,...&Zhang Q..(2014).Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus (SCI).Bmc Microbiology,14(10).
MLA	Zhao Z. X.,et al."Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus (SCI)".Bmc Microbiology 14.10(2014).