Electrochemical DNA Biosensor Based on a Tetrahedral Nanostructure Probe for the Detection of Avian Influenza A (H7N9) Virus

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	Electrochemical DNA Biosensor Based on a Tetrahedral Nanostructure Probe for the Detection of Avian Influenza A (H7N9) Virus
	Dong, SB; Zhao, RT; Zhu, JG; Lu, X; Li, Y; Qiu, SF; Jia, LL; Jiao, X; Song, SP; Fan, CH
刊名	ACS APPLIED MATERIALS & INTERFACES
	2015
卷号	7 期号:16 页码:8834—8842
关键词	GOLD ELECTRODES LABEL-FREE HYBRIDIZATION SEQUENCE PLATFORM SENSOR GENE LNA IMMOBILIZATION AMPLIFICATION
英文摘要	A DNA tetrahedral nanostructure-based electrochemical biosensor was developed to detect avian influenza A (H7N9) virus through recognizing a fragment of the hemagglutinin gene sequence. The DNA tetrahedral probe was immobilized onto a gold electrode surface based on self-assembly between three thiolated nucleotide sequences and a longer nucleotide sequence containing complementary DNA to hybridize with the target single-stranded (ss)DNA. The captured target sequence was hybridized with a biotinylated-ssDNA oligonucleotide as a detection probe, and then avidin-horseradish peroxidase was introduced to produce an amperometric signal through the interaction with 3,3',5,5'-tetramethylbenzidine substrate. The target ssDNA was obtained by asymmetric polymerase chain reaction (PCR) of the cDNA template, reversely transcribed from the viral lysate of influenza A (H7N9) virus in throat swabs. The results showed that this electrochemical biosensor could specifically recognize the target DNA fragment of influenza A (H7N9) virus from other types of influenza viruses, such as influenza A (H1N1) and (H3N2) viruses, and even from single-base mismatches of oligonucleotides. Its detection limit could reach a magnitude of 100 fM for target nucleotide sequences. Moreover, the cycle number of the asymmetric PCR could be reduced below three with the electrochemical biosensor still distinguishing the target sequence from the negative control. To the best of our knowledge, this is the first report of the detection of target DNA from clinical samples using a tetrahedral DNA probe functionalized electrochemical biosensor. It displays that the DNA tetrahedra has a great potential application as a probe of the electrochemical biosensor to detect avian influenza A (H7N9) virus and other pathogens at the gene level, which will potentially aid the prevention and control of the disease caused by such pathogens.
收录类别	SCI
语种	英语
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/24450]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Dong, SB,Zhao, RT,Zhu, JG,et al. Electrochemical DNA Biosensor Based on a Tetrahedral Nanostructure Probe for the Detection of Avian Influenza A (H7N9) Virus[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(16):8834—8842.
APA	Dong, SB.,Zhao, RT.,Zhu, JG.,Lu, X.,Li, Y.,...&Song, HB.(2015).Electrochemical DNA Biosensor Based on a Tetrahedral Nanostructure Probe for the Detection of Avian Influenza A (H7N9) Virus.ACS APPLIED MATERIALS & INTERFACES,7(16),8834—8842.
MLA	Dong, SB,et al."Electrochemical DNA Biosensor Based on a Tetrahedral Nanostructure Probe for the Detection of Avian Influenza A (H7N9) Virus".ACS APPLIED MATERIALS & INTERFACES 7.16(2015):8834—8842.