Determination of Amino Acids in Plasma and Nutritional Supplements by Capillary Electrophoresis with Copper (II) Coordination

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Determination of Amino Acids in Plasma and Nutritional Supplements by Capillary Electrophoresis with Copper (II) Coordination
	Zhou XJ(周贤婧)1,2; Chen J(陈娟)1 ; Shi YP(师彦平)1
刊名	Analytical Letters
	2015
卷号	48 页码:25-36
关键词	Amino acids Capillary electrophoresis Copper(II) Plasma
ISSN号	0003-2719
通讯作者	师彦平
英文摘要	A capillary zone electrophoresis (CZE) method was developed using interactions between amino acids and copper(II) without derivatization. The optimized separation buffer included 10% isopropanol, 50 mmolL^_1 CuSO₄, 50 mmolL^_1 acetic acid, and 50 mmolL^_1 sodium acetate at pH=3.98 at a detection wavelength of 233 nm. This method allowed baseline separation and determination of ten amino acids in less than 21 min. Quality parameters such as linearity (r>0.99), precision (relative standard deviation <2.51%), limits of detection (0.05 lgmL^_1), and recovery (82.25% to 117.6%) were measured. The separation of amino acids in high salt solutions was explored. The method was used to directly determine amino acids in human plasma and nutritional supplements.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Natural Science Foundation of China (Nos. 21105106;21375136)
语种	英语
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/18981]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
作者单位	1.Key Laboratory of Chemistry of Northwestern Plant Resources, CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, P. R. China 2.University of Chinese Academy of Sciences, Beijing, P. R. China
推荐引用方式 GB/T 7714	Zhou XJ,Chen J,Shi YP. Determination of Amino Acids in Plasma and Nutritional Supplements by Capillary Electrophoresis with Copper (II) Coordination[J]. Analytical Letters,2015,48:25-36.
APA	Zhou XJ,Chen J,&Shi YP.(2015).Determination of Amino Acids in Plasma and Nutritional Supplements by Capillary Electrophoresis with Copper (II) Coordination.Analytical Letters,48,25-36.
MLA	Zhou XJ,et al."Determination of Amino Acids in Plasma and Nutritional Supplements by Capillary Electrophoresis with Copper (II) Coordination".Analytical Letters 48(2015):25-36.