Application of preparative chromatography to the isolation of epirubicin from raw product

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Application of preparative chromatography to the isolation of epirubicin from raw product
	Qi, Y; Huang, JX
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
	2001
卷号	24 期号:18 页码:2837-2848
英文摘要	Epirubicin (4'-epidoxorubicin) has been found to be useful in the treatment of cancer. The isolation of milligram amounts of epirubicin from raw product was performed by reversed-phase high-performance liquid chromatography (R-P-HPLC) on Kromasil KR100- 10C(18) (250 x 4.6 mm I.D., 10 mum) column. The purity of epirubicin is required to be greater than 99%. First, is adjusting the mobile phase composition, the column temperature, and the flow-rate to increase the separation factor between the desired component and the nearest neighbor. Then, it is possible to increase the column efficiency and to reduce the time of separation required. Besides, displacement effects could be utilized efficiently to increase the sample size and, therefore, the production rate. The location of the cut points for the collection of target compound was determined by use of detector response levels. This method can be used as a guide to scale up the industrially preparative separation of epirubicin.
WOS记录号	WOS:000171796000008
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/23994]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Qi, Y,Huang, JX. Application of preparative chromatography to the isolation of epirubicin from raw product[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2001,24(18):2837-2848.
APA	Qi, Y,&Huang, JX.(2001).Application of preparative chromatography to the isolation of epirubicin from raw product.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,24(18),2837-2848.
MLA	Qi, Y,et al."Application of preparative chromatography to the isolation of epirubicin from raw product".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 24.18(2001):2837-2848.