Displacement chromatography of isomers and therapeutic compounds

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Displacement chromatography of isomers and therapeutic compounds
	Qi, Y; Huang, JX
刊名	JOURNAL OF CHROMATOGRAPHY A
	2002
卷号	959 期号:2015-01-02 页码:85-93
关键词	displacement chromatography preparative chromatography epirubicin doxorubicin
英文摘要	Displacement chromatography was successfully used to separate a binary isomer mixture, epirubicin and doxorubicin, on Kromasil KR100-10 C-18 250X4.6 mm I.D. (10 mum) column. Displacement parameters such as the types and the concentrations of displacer, the composition and the flow rate of the mobile phase were critically examined in this study. The displacer employed was 30 mg/ml benzethonium chloride. Loading of feed at lower initial organic level of mobile phase coupled with displacement at higher organic level was found to give efficient separation. A 30-mg amount of binary isomer mixture was separated on an analytical column. The purification of epirubicin from the closely related impurities present in raw product solution by displacement chromatography was also investigated. The purity of epirubicin required was greater than 99% with a recovery of 60%. The results have indicated that this process made good use of the high feed load, low solvent costs, and high resolution characteristics of displacement chromatography and offered the chromatographic engineer a powerful tool for the preparative purification of therapeutic compounds. (C) 2002 Elsevier Science B.V. All rights reserved.
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000176608400008
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/23874]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Qi, Y,Huang, JX. Displacement chromatography of isomers and therapeutic compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2002,959(2015-01-02):85-93.
APA	Qi, Y,&Huang, JX.(2002).Displacement chromatography of isomers and therapeutic compounds.JOURNAL OF CHROMATOGRAPHY A,959(2015-01-02),85-93.
MLA	Qi, Y,et al."Displacement chromatography of isomers and therapeutic compounds".JOURNAL OF CHROMATOGRAPHY A 959.2015-01-02(2002):85-93.