Development of a micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) for rapid- and high-throughput analysis of 17 beta-estradiol in water samples

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Development of a micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) for rapid- and high-throughput analysis of 17 beta-estradiol in water samples
	Zhao, LX; Lin, JM
刊名	JOURNAL OF BIOTECHNOLOGY
	2005
卷号	118 期号:2 页码:177-186
关键词	micro-plate magnetic separator micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) 17 beta-estradiol (E2) environmental water sample
英文摘要	A novel method of micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) for the screening of 17 beta-estradiol in water samples was proposed. It used the micro-plate magnetic separator designed by ourselves which can achieve the high-throughput analysis without the samples pre-treatment and the sensitive chemilummescence system of AMPPD-ALP system. The method showed specific recognition of estrogen, without cross-reactions for three other major estrogenic compounds (17 beta-estradiol (E2), estriol (E3), ethinyl (E2)) commonly found in water. The MMCLEIA was also especially suitable for the large-scale samples processing. The working range for 17 beta-estradiol was 10-3000 pg/ml. The assay sensitivity was 5.4 pg/ml. Both intra- and inter-assay had relative standard deviation of less 15%. The effect of several physico-chemical parameters, such as the ratio of antibody versus antigen, incubation time and the concentration of detergent were studied. This method has been successfully applied to the preliminary detection of the sea-water. Compared with the chemiluminescence enzyme-linked immumosorbed assay, the correlation was good. (C) 2005 Elsevier B.V. All rights reserved.
学科主题	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
WOS记录号	WOS:000230580300006
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/23170]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhao, LX,Lin, JM. Development of a micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) for rapid- and high-throughput analysis of 17 beta-estradiol in water samples[J]. JOURNAL OF BIOTECHNOLOGY,2005,118(2):177-186.
APA	Zhao, LX,&Lin, JM.(2005).Development of a micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) for rapid- and high-throughput analysis of 17 beta-estradiol in water samples.JOURNAL OF BIOTECHNOLOGY,118(2),177-186.
MLA	Zhao, LX,et al."Development of a micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) for rapid- and high-throughput analysis of 17 beta-estradiol in water samples".JOURNAL OF BIOTECHNOLOGY 118.2(2005):177-186.