Evaluation of Clostridium ljungdahlii DSM 13528 reference genes in gene expression studies by qRT-PCR | |
Liu, Juanjuan1,2; Tan, Yang2; Yang, Xiaohong1; Chen, Xiaohua2; Li, Fuli2 | |
刊名 | JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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2013-10-01 | |
卷号 | 116期号:4页码:460-464 |
关键词 | Clostridium ljungdahlii Quantitative real-time PCR Reference genes Carbon source Stress |
英文摘要 | Clostridium ljungdahlii DSM 13528 is a promising platform organism for biofuel production from syngas. Gene expression analysis permits a better understanding of the important molecular biological characteristics of this organism, such as carbon fixation and solvent adaptation. Normalization is a prerequisite for accurate gene expression analysis, but until now, no valid reference genes have been proposed for quantitative real-time polymerase chain reaction (qRT-PCR) analysis of C. ljungdahlii DSM 13528. In this study, seven candidate reference genes (gyrA, rho, fotl, rpoA, gukI, recA, 165 rRNA) were selected for qRT-PCR quantification of their expression levels in various culture conditions that corresponded to different carbon sources and stresses. Two analytical programs, geNorm and NormFinder, were used to evaluate reference gene stability. The results showed that gyrA, rho and fotl exhibited the most stable expression levels across all tested samples and can be confidently used as reference genes to normalize the transcriptional data of target genes in qRT-PCR analyses of C. ljungdahlii DSM 13528. This study presents the first attempt to explore the validity of candidate reference genes and provide a set of valid reference genes for normalizing C. ljungdahlii DSM 13528 target gene expression and transcriptome analysis. (C) 2013, The Society for Biotechnology, Japan. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology ; Food Science & Technology |
研究领域[WOS] | Biotechnology & Applied Microbiology ; Food Science & Technology |
关键词[WOS] | REAL-TIME PCR ; APPROPRIATE REFERENCE GENES ; REVERSE-TRANSCRIPTION-PCR ; QUANTITATIVE PCR ; ACETATE PRODUCTION ; SYNTHESIS-GAS ; RT-PCR ; IDENTIFICATION ; FERMENTATION ; VALIDATION |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000326559500008 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn/handle/337004/6029] ![]() |
专题 | 青岛生物能源与过程研究所_微生物资源团队 |
作者单位 | 1.Southwest Univ, Coll Hort & Landscape Architecture, Key Lab Hort Sci So Mountainous Reg, Chongqing 400716, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Juanjuan,Tan, Yang,Yang, Xiaohong,et al. Evaluation of Clostridium ljungdahlii DSM 13528 reference genes in gene expression studies by qRT-PCR[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2013,116(4):460-464. |
APA | Liu, Juanjuan,Tan, Yang,Yang, Xiaohong,Chen, Xiaohua,&Li, Fuli.(2013).Evaluation of Clostridium ljungdahlii DSM 13528 reference genes in gene expression studies by qRT-PCR.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,116(4),460-464. |
MLA | Liu, Juanjuan,et al."Evaluation of Clostridium ljungdahlii DSM 13528 reference genes in gene expression studies by qRT-PCR".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 116.4(2013):460-464. |
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