Characterization of D-xylonate dehydratase YjhG from Escherichia coli

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Characterization of D-xylonate dehydratase YjhG from Escherichia coli
	Jiang, Yudong 1,2; Liu, Wei 1,2; Cheng, Tao 1; Cao, Yujin 1; Zhang, Rubing 1,2; Xian, Mo1
刊名	BIOENGINEERED
	2015-07-01
卷号	6 期号:4 页码:227-232
关键词	biosynthesis D-xylonate dehydratase Escherichia coli YjhG 1 2 4-butanetriol
英文摘要	D-xylonate dehydratase YjhG from Escherichia coli can convert D-xylonate into 2-keto-3-deoxy- D-xylonate (KDX), and is a key enzyme in the biosynthesis of 1,2,4-butanetriol and other chemicals. However, the biochemical properties of YjhG still remain unknown. In this study, the activity assay method for YjhG was established based on semicarbazide method, in which KDX reacts with semicarbazide reagent, and is further quantified by high-resolution mass spectrometry. The effect of reaction conditions on YjhG activity was determined in vitro using purified His-tagged YjhG protein. This enzyme showed maximal activity at 30 degrees C and pH 8.0. Bivalent metal ions such as Mg2+ and Mn2+ activated, whereas Ni2+ and Zn2+ inhibited the activity of YjhG. Under optimal conditions, the K-m and V-max values were 4.88 mM and 78.62 mu M l(-1)h(-1), respectively, when using D-xylonate as a substrate. Amino acids sequence alignments and catalytic properties analysis revealed that YjhG might be a member of IlvD/EDD family. Results obtained in this study may lay a foundation for further investigation on YjhG and will benefit its application in biosynthesis of related chemicals.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	D-XYLOSE ; METABOLISM ; ACID
收录类别	SCI
语种	英语
WOS记录号	WOS:000358662300007
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6521]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Yudong,Liu, Wei,Cheng, Tao,et al. Characterization of D-xylonate dehydratase YjhG from Escherichia coli[J]. BIOENGINEERED,2015,6(4):227-232.
APA	Jiang, Yudong,Liu, Wei,Cheng, Tao,Cao, Yujin,Zhang, Rubing,&Xian, Mo.(2015).Characterization of D-xylonate dehydratase YjhG from Escherichia coli.BIOENGINEERED,6(4),227-232.
MLA	Jiang, Yudong,et al."Characterization of D-xylonate dehydratase YjhG from Escherichia coli".BIOENGINEERED 6.4(2015):227-232.