Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E-coli

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E-coli
	Yang, Jianming 1; Xian, Mo1 ; Su, Sizheng 2; Zhao, Guang 1; Nie, Qingjuan 3; Jiang, Xinglin 1; Zheng, Yanning 1; Liu, Wei 1
刊名	PLOS ONE
	2012-04-27
卷号	7 期号:4
英文摘要	The depleting petroleum reserve, increasingly severe energy crisis, and global climate change are reigniting enthusiasm for seeking sustainable technologies to replace petroleum as a source of fuel and chemicals. In this paper, the efficiency of the MVA pathway on isoprene production has been improved as follows: firstly, in order to increase MVA production, the source of the "upper pathway'' which contains HMG-CoA synthase, acetyl-CoA acetyltransferase and HMG-CoA reductase to covert acetyl-CoA into MVA has been changed from Saccharomyces cerevisiae to Enterococcus faecalis; secondly, to further enhance the production of MVA and isoprene, a alanine 110 of the mvaS gene has been mutated to a glycine. The final genetic strain YJM25 containing the optimized MVA pathway and isoprene synthase from Populus alba can accumulate isoprene up to 6.3 g/L after 40 h of fed-batch cultivation.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	METHYLERYTHRITOL PHOSPHATE-PATHWAY ; HMG-COA SYNTHASE ; MEVALONATE PATHWAY ; ISOPENTENYL DIPHOSPHATE ; BIOSYNTHESIS ; BACTERIA ; AMORPHA-4,11-DIENE ; INTEGRATION ; BIOREACTOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000305336000005
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6383]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Ctr Biomat, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 2.China Risun Coal Chem Grp Ltd, Beijing Risun Chem Technol Inst, Dept Biochem, Beijing, Peoples R China 3.Qingdao Agr Univ, Foreign Languages Sch, Coll English Off, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Yang, Jianming,Xian, Mo,Su, Sizheng,et al. Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E-coli[J]. PLOS ONE,2012,7(4).
APA	Yang, Jianming.,Xian, Mo.,Su, Sizheng.,Zhao, Guang.,Nie, Qingjuan.,...&Liu, Wei.(2012).Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E-coli.PLOS ONE,7(4).
MLA	Yang, Jianming,et al."Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E-coli".PLOS ONE 7.4(2012).