Enhanced production of intracellular dextran dextrinase from Gluconobacter oxydans using statistical experimental methods | |
Mao, Xiangzhao2; Liang, Xiaotong1,2; Wang, Shu2; Dong, Wei1,2; Xing, Yanlong2; Wang, Hualei2; Guo, Lizhong1; Wei, Dongzhi2,3 | |
刊名 | AFRICAN JOURNAL OF BIOTECHNOLOGY
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2010-02-22 | |
卷号 | 9期号:8页码:1180-1189 |
关键词 | Dextran dextrinase Gluconobacter oxydans medium optimization Plackett-Burman design central composite design |
英文摘要 | Optimization of the fermentation medium for DDase production by Gluconaobacter oxydans M5 was carried out in the shake flasks using two kinds of statistical methods. Four variables, namely glucose, tryptone, yeast extract and sodium chloride, were found to influence DDase production significantly by the Plackett-Burman screening. A four-factor five-level central composite design (CCD) was chosen to explain the combined effects of the four medium constituents. The optimum medium consisted of glucose (17.670 g/L), maltobiose (30 g/L), tryptone (12.198 g/L), yeast extract (13.528 g/L), ammonium nitrate (15 g/L), copper sulfate (0.01 g/L), zinc sulfate (0.01 g/L), and sodium chloride (0.009 g/L); the initial pH 6.0 was set prior to sterilization. The DDase yield obtained from optimized medium increased by 17-fold (0.238 U/mL) or so. Under these optimal conditions, the experimental values agreed with the predicted values, indicating that the chosen method of optimization of medium composition was efficient, relatively simple, time reducing and material saving. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology |
关键词[WOS] | CAPSULATUS ATCC 11894 ; PURIFICATION ; MECHANISM |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000275382400011 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn/handle/337004/6634] ![]() |
专题 | 青岛生物能源与过程研究所_生物反应工程团队 |
作者单位 | 1.Qingdao Agr Univ, Shandong Prov Key Lab Appl Mycol, Qingdao, Peoples R China 2.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Beijing 100864, Peoples R China 3.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China |
推荐引用方式 GB/T 7714 | Mao, Xiangzhao,Liang, Xiaotong,Wang, Shu,et al. Enhanced production of intracellular dextran dextrinase from Gluconobacter oxydans using statistical experimental methods[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(8):1180-1189. |
APA | Mao, Xiangzhao.,Liang, Xiaotong.,Wang, Shu.,Dong, Wei.,Xing, Yanlong.,...&Wei, Dongzhi.(2010).Enhanced production of intracellular dextran dextrinase from Gluconobacter oxydans using statistical experimental methods.AFRICAN JOURNAL OF BIOTECHNOLOGY,9(8),1180-1189. |
MLA | Mao, Xiangzhao,et al."Enhanced production of intracellular dextran dextrinase from Gluconobacter oxydans using statistical experimental methods".AFRICAN JOURNAL OF BIOTECHNOLOGY 9.8(2010):1180-1189. |
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