Simultaneously improving stability and specificity of cell surface displayed glucose dehydrogenase mutants to construct whole-cell biocatalyst for glucose biosensor application

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 生物传感技术团队

	Simultaneously improving stability and specificity of cell surface displayed glucose dehydrogenase mutants to construct whole-cell biocatalyst for glucose biosensor application
	Liang, Bo ; Lang, Qiaolin ; Tang, Xiangjiang ; Liu, Aihua 1
刊名	BIORESOURCE TECHNOLOGY
	2013-11-01
卷号	147 页码:492-498
关键词	Glucose dehydrogenase mutant Surface display Enzyme stability Substrate specificity Glucose biosensor
英文摘要	The improved stability and substrate specificity of cell surface displayed glucose dehydrogenase (GDH) mutants by replacing four amino acids from Bacillus subtilis by using site-directed mutagenesis was systematically investigated. A series of mutated GDHs including E170R/Q252L, V149K/E170R/Q252L, E170R/Q252L/G259A and V149K/E170R/Q252L/G259A, were fused to the ice nucleation protein for displaying on cell surface of Eschericia coli. Q252L/E170R/V149K, Q252L/E170R/G259A and Q252L/E170R/V149K/G259A variants were found stable at a wide pH range and shown excellent thermostability. Especially, the Q252L/E170R/V149K/G259A mutant showed half-life of similar to 3.8 days at 70 degrees C. Q252L/E170R/V149K/G259A variant exhibited the narrowest substrate specificity for D-glucose. The whole cell displayed GDH mutant could be cultured in a large scale with excellent enzyme activity and productivity. In addition, a sensitive and stable electrochemical glucose biosensor can be prepared using the GDH-mutant bacteria modified electrode. Thus, the whole cell biocatalysts are promising candidates for exploitation in a wide range of industrial applications. (C) 2013 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	BACILLUS-MEGATERIUM IWG3 ; D-XYLOSE ; INCREASING MUTANTS ; MODIFIED-ELECTRODE ; ESCHERICHIA-COLI ; BIOFUEL CELL ; FUEL-CELLS ; BACTERIA ; ENZYMES ; PROTEINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000326660900067
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6020]
专题	青岛生物能源与过程研究所_生物传感技术团队
作者单位	1.Chinese Acad Sci, Lab Biosensing, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Liang, Bo,Lang, Qiaolin,Tang, Xiangjiang,et al. Simultaneously improving stability and specificity of cell surface displayed glucose dehydrogenase mutants to construct whole-cell biocatalyst for glucose biosensor application[J]. BIORESOURCE TECHNOLOGY,2013,147:492-498.
APA	Liang, Bo,Lang, Qiaolin,Tang, Xiangjiang,&Liu, Aihua.(2013).Simultaneously improving stability and specificity of cell surface displayed glucose dehydrogenase mutants to construct whole-cell biocatalyst for glucose biosensor application.BIORESOURCE TECHNOLOGY,147,492-498.
MLA	Liang, Bo,et al."Simultaneously improving stability and specificity of cell surface displayed glucose dehydrogenase mutants to construct whole-cell biocatalyst for glucose biosensor application".BIORESOURCE TECHNOLOGY 147(2013):492-498.