Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 酶工程团队

	Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid
	Zhang, Wei 1; Cao, Shaona 1; Qiu, Li 2; Qi, Fengxia 1; Li, Zhong 1,3; Yang, Ying 1; Huang, Shaohua 1; Bai, Fali 1; Liu, Changning 4; Wan, Xiaobo 2
刊名	CHEMBIOCHEM
	2015-03-02
卷号	16 期号:4 页码:565-569
关键词	biosynthesis methyltransferases mycophenolic acid substrate flexibility transferases
英文摘要	Mycophenolic acid (MPA, 1) is a clinically important immunosuppressant. In this report, a gene cluster mpa responsible for the biosynthesis of 1 was identified from Penicillium brevicompactum NRRL 864. The S-adenosyl-L-methionine-dependent (SAM-dependent) O-methyltransferase encoded by the mpaG gene was functionally and kinetically characterized in vitro. MpaG catalyzes the methylation of demethylmycophenolic acid (DMMPA, 6) to form 1. It also showed significant substrate flexibility by methylating two structural derivatives of 6 prepared by organic synthesis.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Medicinal
研究领域[WOS]	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]	PENICILLIUM-BREVICOMPACTUM ; DEHYDROGENASE ; FUNGI
收录类别	SCI ; IC
语种	英语
WOS记录号	WOS:000350138700005
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6083]
专题	青岛生物能源与过程研究所_酶工程团队
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci Menglun, Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Menglun 666303, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wei,Cao, Shaona,Qiu, Li,et al. Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid[J]. CHEMBIOCHEM,2015,16(4):565-569.
APA	Zhang, Wei.,Cao, Shaona.,Qiu, Li.,Qi, Fengxia.,Li, Zhong.,...&Li, Shengying.(2015).Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid.CHEMBIOCHEM,16(4),565-569.
MLA	Zhang, Wei,et al."Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid".CHEMBIOCHEM 16.4(2015):565-569.