Genomic and transcriptome analyses reveal that MAPK- and phosphatidylinositol-signaling pathways mediate tolerance to 5-hydroxymethyl-2-furaldehyde for industrial yeast Saccharomyces cerevisiae

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 单细胞中心

	Genomic and transcriptome analyses reveal that MAPK- and phosphatidylinositol-signaling pathways mediate tolerance to 5-hydroxymethyl-2-furaldehyde for industrial yeast Saccharomyces cerevisiae
	Zhou, Qian 1,2; Liu, Z. Lewis 3; Ning, Kang 1,2; Wang, Anhui 4; Zeng, Xiaowei 1,2; Xu, Jian1,2
刊名	SCIENTIFIC REPORTS
	2014-10-09
卷号	4
英文摘要	The industrial yeast Saccharomyces cerevisiae is a traditional ethanologenic agent and a promising biocatalyst for advanced biofuels production using lignocellulose mateials. Here we present the genomic background of type strain NRRL Y-12632 and its transcriptomic response to 5-hydroxymethyl-2-furaldehyde (HMF), a commonly encountered toxic compound liberated from lignocellulosic-biomass pretreatment, in dissecting the genomic mechanisms of yeast tolerance. Compared with the genome of laboratory model strain S288C, we identified more than 32,000 SNPs in Y-12632 with 23,000 missense and nonsense SNPs. Enriched sequence mutations occurred for genes involved in MAPK- and phosphatidylinositol (PI)-signaling pathways in strain Y-12632, with 41 and 13 genes containing non-synonymous SNPs, respectively. Many of these mutated genes displayed consistent up-regulated signature expressions in response to challenges of 30 mM HMF. Analogous single-gene deletion mutations of these genes showed significantly sensitive growth response on a synthetic medium containing 20 mM HMF. Our results suggest at least three MAPK-signaling pathways, especially for the cell-wall integrity pathway, and PI-signaling pathways to be involved in mediation of yeast tolerance against HMF in industrial yeast Saccharomyces cerevisiae. Higher levels of sequence variations were also observed for genes involved in purine and pyrimidine metabolism pathways.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	LIGNOCELLULOSIC BIOMASS CONVERSION ; ETHANOLOGENIC YEAST ; ALDEHYDE REDUCTASE ; GENE-EXPRESSION ; INHIBITORS ; ADAPTATION ; HMF ; DETOXIFICATION ; IDENTIFICATION ; PHYLOGENIES
收录类别	SCI
语种	英语
WOS记录号	WOS:000343060300003
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6242]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 3.USDA ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA 4.China Three Gorges Univ, Coll Comp & Informat Technol, Yichang, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Qian,Liu, Z. Lewis,Ning, Kang,et al. Genomic and transcriptome analyses reveal that MAPK- and phosphatidylinositol-signaling pathways mediate tolerance to 5-hydroxymethyl-2-furaldehyde for industrial yeast Saccharomyces cerevisiae[J]. SCIENTIFIC REPORTS,2014,4.
APA	Zhou, Qian,Liu, Z. Lewis,Ning, Kang,Wang, Anhui,Zeng, Xiaowei,&Xu, Jian.(2014).Genomic and transcriptome analyses reveal that MAPK- and phosphatidylinositol-signaling pathways mediate tolerance to 5-hydroxymethyl-2-furaldehyde for industrial yeast Saccharomyces cerevisiae.SCIENTIFIC REPORTS,4.
MLA	Zhou, Qian,et al."Genomic and transcriptome analyses reveal that MAPK- and phosphatidylinositol-signaling pathways mediate tolerance to 5-hydroxymethyl-2-furaldehyde for industrial yeast Saccharomyces cerevisiae".SCIENTIFIC REPORTS 4(2014).