Raman spectroscopy provides a rapid, non-invasive method for quantitation of starch in live, unicellular microalgae

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 单细胞中心

	Raman spectroscopy provides a rapid, non-invasive method for quantitation of starch in live, unicellular microalgae
	Ji, Yuetong 1,2; He, Yuehui 1,2,3; Cui, Yanbin 4; Wang, Tingting 1,2; Wang, Yun 1,2; Li, Yuanguang 4; Huang, Wei E.5; Xu, Jian1,2
刊名	BIOTECHNOLOGY JOURNAL
	2014-12-01
卷号	9 期号:12 页码:1512-1518
关键词	Microalgae Non-invasive analysis Raman spectroscopy Single-cell analysis Starch
英文摘要	Conventional methods for quantitation of starch content in cells generally involve starch extraction steps and are usually labor intensive, thus a rapid and non-invasive method will be valuable. Using the starch-producing unicellular microalga Chlamydomonas reinhardtii as a model, we employed a customized Raman spectrometer to capture the Raman spectra of individual single cells under distinct culture conditions and along various growth stages. The results revealed a nearly linear correlation (R-2 = 0.9893) between the signal intensity at 478 cm(-1) and the starch content of the cells. We validated the specific correlation by showing that the starch-associated Raman peaks were eliminated in a mutant strain where the AGPase (ADP-glucose pyrophosphorylase) gene was disrupted and consequentially the biosynthesis of starch blocked. Furthermore, the method was validated in an industrial algal strain of Chlorella pyrenoidosa. This is the first demonstration of starch quantitation in individual live cells. Compared to existing cellular starch quantitation methods, this single-cell Raman spectra-based approach is rapid, label-free, non-invasive, culture-independent, low-cost, and potentially able to simultaneously track multiple metabolites in individual live cells, therefore should enable many new applications.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	CHLAMYDOMONAS-REINHARDTII ; CELL ; MICROSPECTROSCOPY ; ACCUMULATION ; BIOFUELS ; MICROSCOPY
收录类别	SCI
语种	英语
WOS记录号	WOS:000346021200011
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6141]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 5.Univ Sheffield, Kroto Inst, Sheffield, S Yorkshire, England
推荐引用方式 GB/T 7714	Ji, Yuetong,He, Yuehui,Cui, Yanbin,et al. Raman spectroscopy provides a rapid, non-invasive method for quantitation of starch in live, unicellular microalgae[J]. BIOTECHNOLOGY JOURNAL,2014,9(12):1512-1518.
APA	Ji, Yuetong.,He, Yuehui.,Cui, Yanbin.,Wang, Tingting.,Wang, Yun.,...&Xu, Jian.(2014).Raman spectroscopy provides a rapid, non-invasive method for quantitation of starch in live, unicellular microalgae.BIOTECHNOLOGY JOURNAL,9(12),1512-1518.
MLA	Ji, Yuetong,et al."Raman spectroscopy provides a rapid, non-invasive method for quantitation of starch in live, unicellular microalgae".BIOTECHNOLOGY JOURNAL 9.12(2014):1512-1518.