Anti-apoptotic activity of Bak Foong Pills and its ingredients on 6-hydroxydopamine-induced neurotoxicity in PC12 cells

	Anti-apoptotic activity of Bak Foong Pills and its ingredients on 6-hydroxydopamine-induced neurotoxicity in PC12 cells
	Jia, RR ; Gou, YL ; Ho, LS ; Ng, CP ; Tan, NH ; Chan, HC
刊名	CELL BIOLOGY INTERNATIONAL
	2005-10-01
卷号	29 期号:10 页码:835-842
关键词	Bak Foong Pills (BFP) 6-hydroxydopamine (6-OHDA) PC12 cell apoptosis Parkinson's disease
英文摘要	Bak Foong Pills (BFP), a traditional Chinese medicine used for centuries for the enhancement of women's health, was shown to display neuro -protective activity in the 1-methyl-4-phenyl-1,2,4,6,-tetrahydro-pyridine (MPTP)-induced mouse model in a previous study. In order to elucidate its mechanism of action, we investigated the anti-apoptotic properties of Bak Foong Pills and its main ingredients, including Panax ginseng, Angelica sinensis, Glycyrrhiza uralensis, and Ligusticum chuanxiong, in the 6-hydroxydopamine (6-OHDA)-treated PC12 cell model. The addition of the neurotoxin could cause significant cell death and reduction of cell proliferation, as shown in the results determined by MTT assay, nitric oxide (NO) measurement and flow cytometric propidium iodine (PI) staining analysis, while pre-treatment of PC 12 cell with either BFP or its main ingredients prevented the toxicity to some degree. In addition, the neurotoxin caused an elevated activation of caspase-3, the key enzyme for activation of the cellular apoptotic cascade, whereas BFP or its main ingredients inhibited the activation of caspase-3. These results strongly indicate that BFP and its main ingredients may provide a useful therapeutic strategy for the treatment of neurodegenerative diseases, such as Parkinson's disease. (c) 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
类目[WOS]	Cell Biology
研究领域[WOS]	Cell Biology
关键词[WOS]	NITRIC-OXIDE SYNTHASE ; PARKINSONS-DISEASE ; NEURODEGENERATIVE DISORDERS ; MEDIATED APOPTOSIS ; OXIDATIVE STRESS ; PATHOGENESIS ; GLUTAMATE ; LIPOPOLYSACCHARIDE ; ACTIVATION ; INDUCTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000232508000001
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/24790]
专题	昆明植物研究所_离退休
作者单位	1.Chinese Univ Hong Kong, Dept Physiol, Epithelial Cell Biol Res Ctr, Shatin, Hong Kong, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Jia, RR,Gou, YL,Ho, LS,et al. Anti-apoptotic activity of Bak Foong Pills and its ingredients on 6-hydroxydopamine-induced neurotoxicity in PC12 cells[J]. CELL BIOLOGY INTERNATIONAL,2005,29(10):835-842.
APA	Jia, RR,Gou, YL,Ho, LS,Ng, CP,Tan, NH,&Chan, HC.(2005).Anti-apoptotic activity of Bak Foong Pills and its ingredients on 6-hydroxydopamine-induced neurotoxicity in PC12 cells.CELL BIOLOGY INTERNATIONAL,29(10),835-842.
MLA	Jia, RR,et al."Anti-apoptotic activity of Bak Foong Pills and its ingredients on 6-hydroxydopamine-induced neurotoxicity in PC12 cells".CELL BIOLOGY INTERNATIONAL 29.10(2005):835-842.